Application
Western blot analysis of RAIDD expression in non-transfected: (A) and mouse RAIDD transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in granular and molecular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic localization.Application
Western blot analysis of human recombinant RAIDD fusion protein.| Product Name | RAIDD Antibody (FL-199) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-199 representing full length RAIDD of human origin |
| Modification Notes | A cytoplasmic domain of approximately 80 amino acids has been identified in the apoptosis-mediating receptors of TNF-R1 and FAS. This region was determined to be necessary for the transduction of the apoptotic signal and was designated the “death domain”. Other death domain-containing, but otherwise structurally unrelated, proteins were identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. The receptor interacting protein RIP is a death domain-containing serine/threonine kinase which associates with FAS or the TNF-R1 binding protein TRADD. RAIDD (RIP-associated ICH-1/Ced-3 homologous protein with a death domain) has been identified as a RIP binding protein that also associates with members of the caspase family, providing a link between activation of the TNF-Rs and the triggering of the cysteine protease cascade. The amino-terminal domain of RAIDD shares significant homology with the prodomain of ICH-1 and mediates the binding of RAIDD to this cysteine protease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RAIDD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-199 representing full length RAIDD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-199 representing full length RAIDD of human origin |
| Entrez Gene | 8738 |
|---|
Application
Western blot analysis of RAIDD expression in non-transfected: (A) and mouse RAIDD transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in granular and molecular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic localization.
Application
Western blot analysis of human recombinant RAIDD fusion protein.| Application Notes | recommended for detection of RAIDD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
