Application
Western blot analysis of AIF expression in non-transfected 293T: (A), human AIF transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and nuclear staining.Application
Western blot analysis of AIF expression in AML-193 (A), CCRF-CEM (B), Hep G2 (C) and MOLT-4 (D) whole cell lysates.| Product Name | AIF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIF of human origin |
| Modification Notes | A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AIF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of AIF of human origin |
| Entrez Gene | 9131 |
|---|
Application
Western blot analysis of AIF expression in non-transfected 293T: (A), human AIF transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and nuclear staining.
Application
Western blot analysis of AIF expression in AML-193 (A), CCRF-CEM (B), Hep G2 (C) and MOLT-4 (D) whole cell lysates.| Application Notes | recommended for detection of AIF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
