Application
Western blot analysis of rat recombinant PAR4.Application
Western blot analysis of PAR4 expression in non-transfected 293: (A), mouse PAR4 transfected 293: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of PAR4 expression in AT-3 whole cell lysate (A) and 20 ng PAR4 polyhistidine fusion protein (B).| Product Name | PAR4 Antibody (R-334) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-334 representing full length PAR4 of rat origin |
| Modification Notes | Normal tissues are characterized by a balance between cellular stasis, cell proliferation, cell differentiation and cell death. Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PAR4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-334 representing full length PAR4 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-334 representing full length PAR4 of rat origin |
| Entrez Gene | 5074 |
|---|
Application
Western blot analysis of rat recombinant PAR4.
Application
Western blot analysis of PAR4 expression in non-transfected 293: (A), mouse PAR4 transfected 293: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of PAR4 expression in AT-3 whole cell lysate (A) and 20 ng PAR4 polyhistidine fusion protein (B).| Application Notes | recommended for detection of PAR4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
