Application
Western blot analysis of APC16 expression in non-transfected: (A) and mouse APC16 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of APC16 expression in non-transfected: (A) and human APC16 transfected: (B) 293T whole cell lysates.| Product Name | APC16 Antibody (C-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of APC16 of human origin |
| Modification Notes | APC16, also known as ANAPC16 (anaphase promoting complex subunit 16), MSAG, cyclosome subunit 16 or metabolic syndrome-associated protein, is a 110 amino acid protein belonging to the APC16 family. Encoded by a gene that maps to human chromosome 10q22.1, APC16 contains four putative myristoylation sites and three phosphorylation sites. Highly conserved among mammalian species, APC16 of human, orangutan, monkey, mouse, horse and dog origin are identical. APC16 is a component of the anaphase promoting complex/cyclosome (APC/C), which is a cell cycle-regulated E3 ubiquitin ligase that controls cell cycle progression through mitosis and G1. The APC/C complex mediates ubiquitination and degradation of target proteins. Composed of a minimum of twelve subunits, APC/C largely assists Lys-11-linked polyubiquitin chain formation, as well as Lys-48- and Lys-63-linked polyubiquitin chain formation. APC16 associates with APC/C independently of ANAPC2 and APC11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APC16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of APC16 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of APC16 of human origin |
| Entrez Gene | 119504 |
|---|
Application
Western blot analysis of APC16 expression in non-transfected: (A) and mouse APC16 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of APC16 expression in non-transfected: (A) and human APC16 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of APC16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
