Application
Western blot analysis of PLC β3 phosphorylation in non-transfected: (A) and mouse PLC β3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PLC β3 phosphorylation in KNRK whole cell lysate.Application
Western blot analysis of PLC β3 phosphorylation in non-transfected: (A) and human PLC β3 transfected: (B) 293T whole cell lysates.| Product Name | p-PLC β3 Antibody (Ser 537) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated within an internal region of PLC β3 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1 and PLC ∂2). The ©-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two ©-type enzymes, but not the ∫ and ∂ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC ∫1, PLC ∫2 and PLC ∫3 is mediated by the a subunits of the Gq class of heterotrimeric G proteins and by certain bg G protein subunits. PLC ∫3 is phosphorylated on Ser 537 in the basal state in cells, and this phosphorylation is enhanced by ionomycin. PLC ∫3 is also exclusively phosphorylated on Ser 537 in the X-Y linker region by CaMK II. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated within an internal region of PLC &beta 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated within an internal region of PLC β3 of human origin |
| Entrez Gene | 5331 |
|---|
Application
Western blot analysis of PLC β3 phosphorylation in non-transfected: (A) and mouse PLC β3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PLC β3 phosphorylation in KNRK whole cell lysate.
Application
Western blot analysis of PLC β3 phosphorylation in non-transfected: (A) and human PLC β3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
