Application
Western blot analysis of DPYD expression in HL-60 whole cell lysate.Application
Western blot analysis of DPYD expression in non-transfected: (A) and mouse DPYD transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DPYD expression in non-transfected 293T: (A), human DPYD transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | DPYD Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DPYD of human origin |
| Modification Notes | Dihydropyrimidine dehydrogenase (DPYD) catalyzes the first rate-limiting step of the NADPH-dependent catabolism of uracil and thymine to dihydrouracil and dihydrothymine; thus, a deficiency of DPYD leads to an accumulation of uracil and thymine. Abnormal concentrations of these metabolites in bodily fluids may be the cause of neurological disease and a contraindication for treatment of cancer patients with certain pyrimidine analogs. DPYD also catalyzes the anticancer agent 5-fluorouracil (5-FU) pathway and is involved in the efficacy and toxicity of 5-FU. Variations in DPYD concentration may arise from alterations at the transcriptional level of the dihydropyrimidine dehydrogenase gene. Specifically, hypermethylation of the DPYD promoter downregulates dihydropyrimidine dehydrogenase expression. Deficient DPYD alleles may constitute a risk factor for severe toxicity following treatment with 5-FU. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DPYD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DPYD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DPYD of human origin |
| Entrez Gene | 1806 |
|---|
Application
Western blot analysis of DPYD expression in HL-60 whole cell lysate.
Application
Western blot analysis of DPYD expression in non-transfected: (A) and mouse DPYD transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DPYD expression in non-transfected 293T: (A), human DPYD transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of DPYD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
