Application
Western blot analysis of MRM1 expression in non-transfected: (A) and human MRM1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MRM1 expression in non-transfected: (A) and human MRM1 transfected: (B) 293T whole cell lysates.| Product Name | MRM1 Antibody (C-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of MRM1 of human origin |
| Modification Notes | MRM1 (mitochondrial rRNA methyltransferase 1) is a 353 amino acid protein that localizes to the mitochondrion and belongs to the RNA methyltransferase trmH family. Expressed as two alternatively spliced isoforms, MRM1 specifically methylates the ribose of guanosine G-2270 in the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S). The gene encoding MRM1 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MRM1 isoforms 1 and 2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of MRM1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of MRM1 of human origin |
| Entrez Gene | 79922 |
|---|
Application
Western blot analysis of MRM1 expression in non-transfected: (A) and human MRM1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MRM1 expression in non-transfected: (A) and human MRM1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MRM1 isoforms 1 and 2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
