Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of CYP1B1 expression in non-transfected: (A) and human CYP1B1 transfected: (B) 293T whole cell lysates.| Product Name | CYP1B1 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 221-325 mapping within an internal region of CYP1B1 of human origin |
| Modification Notes | Cytochrome P450 1B1 (CYP1B1) is a key enzyme involved in the production of potentially carcinogenic estrogen metabolites and the activation of environmental carcinogens and is the predominant member of the CYP1 family expressed in normal breast tissue and breast cancer. Estrogen has been proposed to trigger breast cancer development via an initiating mechanism involving its metabolite, catechol estrogen (CE). CYP1B1 catalyzes the conversion of 17-beta-estradiol to the catechol estrogen metabolites 2-OH-E2 and 4-OH-E2 which have both been postulated to be involved in mammary carcinogenesis. Genetic polymorphisms in CYP1B1 may play an important role in human prostate carcinogenesis as well. Polymorphism of the CYP1B1 gene at codon 432 (Val-->Leu) is associated with a change in catalytic function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CYP1B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-325 mapping within an internal region of CYP1B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-325 mapping within an internal region of CYP1B1 of human origin |
| Entrez Gene | 1545 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of CYP1B1 expression in non-transfected: (A) and human CYP1B1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CYP1B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
