| Product Name | APIP Antibody (H-72) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 171-242 mapping at the C-terminus of APIP of human origin |
| Modification Notes | APIP (Apaf-1-interacting protein), also known as APIP2, is a member of the aldolase class II family and has a highly conserved C-terminal from C. elegans to humans. It is ubiquitously expressed, with high expression levels in heart, kidney and skeletal muscle. Alternative splicing produces two isoforms of APIP. Isoform 1 is the full length, 242 amino acid protein; isoform 2 is missing residues 1-38 and contains a distinct sequence for amino acids 39-53. APIP plays an important role in preventing muscle ischemic damage. It suppresses hypoxia-induced cell death by inducing the activation of Akt and ERK 1/2, which are responsible for inhibition of caspase-9 via phosphorylation, and competing with caspase-9 to bind the caspase recruitment domain (CARD) of Apaf-1. Through these mechanisms, APIP negatively regulates the activation of caspase-9 and Apaf-1-mediated cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 171-242 mapping at the C-terminus of APIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 171-242 mapping at the C-terminus of APIP of human origin |
| Entrez Gene | 51074 |
|---|
| Application Notes | recommended for detection of APIP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
