Application
Western blot analysis of SH3D19 expression in Hep G2 whole cell lysate.| Product Name | SH3D19 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 441-740 mapping near the C-terminus of SH3D19 of human origin |
| Modification Notes | SH3D19 (SH3 domain-containing protein 19), also known as EBP, EEN-binding protein, EVE1 or Kryn, is a 790 amino acid protein that is widely expressed with highest expression in kidney, heart, lung, liver, skeletal muscle and small intestine. Localized to the cytoplasm and recruited to the nucleus by a fusion protein known as MLL-EEN (mixed-lineage leukemia-endophilin), SH3D19 is thought to play a role in regulating the involvement of ADAM (A disintegrin and metalloproteases) proteins in EGFR (epidermal growth factor receptor)-ligand shedding pathways. SH3D19 contains five SH3 domains and may also function to suppress both Ras-induced cellular transformation and Ras-associated activation of proteins such as Elk-1. Translocation events involving the SH3D19 gene are implicated in acute myeloid leukemia, suggesting a possible role for SH3D19 in carcinogenesis. Five isoforms of SH3D19 are expres-sed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SH3D19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 441-740 mapping near the C-terminus of SH3D19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 441-740 mapping near the C-terminus of SH3D19 of human origin |
| Entrez Gene | 152503 |
|---|
Application
Western blot analysis of SH3D19 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of SH3D19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
