Application
Western blot analysis of LYPLA3 expression in HeLa (A) and K-562 (B) whole cell lysates.| Product Name | LYPLA3 Antibody (H-167) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 246-412 mapping at the C-terminus of LYPLA3 of human origin |
| Modification Notes | LYPLA3 (lysophospholipase 3) also referred to as ACS, LLPL or LPLA2 (lysosomal phospholipase A2), is ubiquitously expressed with highest expression in kidney, placenta, pancreas, testis, spleen, heart and skeletal muscle. LYPLA3, which localizes to lysosomes, is calcium-independent and has an acidic pH optimum. LYPLA3 transacylates ceramide and increases lysosomal membrane permeability to both potassium ions and protons. It may play a critical role in pulmonary surfactant phospholipid degradation due to its high specificity towards phosphatidylethanolamine and phosphatidylcholine in alveolar macro-phages. LYPLA3 may also enhance lysosome osmotic sensitivity, resulting in the destabilization of the enzyme by causing leakage and inducing apoptosis. LYPLA3 is thought to remodel acyl groups and modulate the biological and pharmacological activities of some lipophilic alcohols. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LYPLA3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 246-412 mapping at the C-terminus of LYPLA3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 246-412 mapping at the C-terminus of LYPLA3 of human origin |
| Entrez Gene | 23659 |
|---|
Application
Western blot analysis of LYPLA3 expression in HeLa (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of LYPLA3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
