Application
Western blot analysis of HERG expression in EOC 20 whole cell lysate.Application
Western blot analysis of HERG expression in EOC 20 (A) and EOC 13.31 (B) whole cell lysates.| Product Name | HERG Antibody (H-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 96-270 mapping near the N-terminus of HERG of human origin |
| Modification Notes | Human ether-a-go-go related gene (HERG) encodes the pore-forming å subunit of the delayed rectifier potassium channel IKr. The HERG subunit contains six transmembrane å-helices with a reentrant “pore-loop” between the fifth and the sixth transmembrane helices. The two N-terminal splice variants of HERG include the full-length isoform 1å and the shorter isoform 1∫. Isoform 1∫ lacks the PAS motif and deactivates at a faster rate than isoform 1å. Residues within the C-terminal play a role in channel expression and channel gating, including voltage-dependent activation. HERG is expressed in the heart and is more abundantly expressed in the ventricles than in the atria. Mutations in the gene encoding HERG increase beat-to-beat variability and early after depolarization. These fluctuations facilitate the genesis and propagation of premature heartbeats associated with inheritable long QT syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HERG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 96-270 mapping near the N-terminus of HERG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 96-270 mapping near the N-terminus of HERG of human origin |
| Entrez Gene | 3757 |
|---|
Application
Western blot analysis of HERG expression in EOC 20 whole cell lysate.
Application
Western blot analysis of HERG expression in EOC 20 (A) and EOC 13.31 (B) whole cell lysates.| Application Notes | recommended for detection of HERG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
