Application
Western blot analysis of ENOPH1 expression in non-transfected 293T: (A), mouse ENOPH1 transfected 293T: (B), HL-60 (C), HeLa (D) and K-562 (E) whole cell lysates.Application
Western blot analysis of ENOPH1 expression in non-transfected: (A) and human ENOPH1 transfected: (B) 293T whole cell lysates.| Product Name | ENOPH1 Antibody (FL-261) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-261 representing full length ENOPH1 of human origin |
| Modification Notes | ENOPH1 (enolase-phosphatase 1), also known as E1, MASA or MST145, is a member of the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. Existing as a monomer and binding magnesium as a cofactor, ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulates methionine levels in the cell (also known as the Yang cycle in plants). More specifically, ENOPH1 catalyzes the continuous enolization and dephosphorylation of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite 1,2-dihydroxy-3-keto-5-methylthiopentene. Due to alternative splicing events, two isoforms exist for ENOPH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ENOPH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-261 representing full length ENOPH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-261 representing full length ENOPH1 of human origin |
Application
Western blot analysis of ENOPH1 expression in non-transfected 293T: (A), mouse ENOPH1 transfected 293T: (B), HL-60 (C), HeLa (D) and K-562 (E) whole cell lysates.
Application
Western blot analysis of ENOPH1 expression in non-transfected: (A) and human ENOPH1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ENOPH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
