Application
Western blot analysis of NF45 expression in Jurkat (A) and K-562 (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.| Product Name | NF45 Antibody (H-185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-205 mapping near the N-terminus of NF45 of human origin |
| Modification Notes | NF45 (ILF2, nuclear factor of activated T cells 45 kDa) is a transcription factor that interacts with NF90 (ILF3, DRBP76) to regulate gene expression. NF45 and NF90 are proteins that belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. The NF45/NF90 heterodimer is mainly involved in regulating IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NF45 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-205 mapping near the N-terminus of NF45 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-205 mapping near the N-terminus of NF45 of human origin |
Application
Western blot analysis of NF45 expression in Jurkat (A) and K-562 (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.| Application Notes | recommended for detection of NF45 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
