| Product Name | Gcn4 Antibody (FL-281) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-281 representing full length Gcn4 of Saccharomyces cerevisiae origin |
| Modification Notes | During amino acid starvation, Saccharomyces cerevisiae utilizes a network of proteins to activate the transcription of amino acid biosynthetic genes (1). Phosphorylation of the eukaryotic initiation factor 2 (eIF-2a) by Gcn2 downregulates total protein synthesis as well as decreases the amount of uncharged tRNA (2,3). Uncharged tRNA recognizes the initiation codon of Gcn4 mRNA to increase levels of the Gcn4 protein, a transcriptional activator of amino acid biosynthetic precursors (2,3). Gcn4 recognizes a specific DNA-binding motif sequence designated the Gcn4-protein responsive element (GCRE), which is present in the promoter regions of its target genes (1). Gcn4 targets include genes in every amino acid biosynthetic pathway except cysteine as well as genes encoding vitamin biosynthetic enzymes, peroxisomal components, mitochondrial carrier proteins, and autophagy proteins (4,5). UV radiation and glucose stimulation also induce Gcn4 activity through the Ras/cAMP pathway (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gcn4 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-281 representing full length Gcn4 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-281 representing full length Gcn4 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Gcn4 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
