Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and human Rab 27a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and mouse Rab 27a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and mouse Rab 27a transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | Rab 27a Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 162-221 mapping at the C-terminus of Rab 27a of human origin |
| Modification Notes | The Rab family of low molecular weight GTPases are critical regulators of vesicular transport (1). Rab proteins cycle between an active GTP-bound state, which recruits specific effector proteins, and an inactive GDP-bound state (1). Two members of this family, Rab27a and Rab27b, have overlapping functions, but differ in tissue specificity (2,3). Rab27a is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain (3). Rab27b is also expressed in pituitary tissue, but is more significantly expressed in brain and spleen (3). Rab27a regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes and lytic granule release in cytotoxic T lymphocytes (4). Mutations in the Rab27a gene result in Griscelli syndrome (GS) or the corresponding mouse model ashen, a rare autosomal recessive disorder characterized by hypopigmentation, prolonged bleeding times, and platelet storage pool deficiency (2,4). In GS, Rab27a is not available to mediate the recruitment of melanosomes via the actin motor, myosin Va (5,6). The human Rab27b gene maps to chromosome 18q21.1, and encodes a protein that is involved in pituitary hormone secretion (3,7). Rab27b may be functionally redundant to Rab27a, as it can rescue Rab27a mutants (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rab27a and, to a lesser extent, Rab 27b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 162-221 mapping at the C-terminus of Rab 27a of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 162-221 mapping at the C-terminus of Rab 27a of human origin |
| Entrez Gene | 5873 |
|---|
Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and human Rab 27a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and mouse Rab 27a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 27a expression in non-transfected: (A) and mouse Rab 27a transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Rab27a and, to a lesser extent, Rab 27b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
