Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of Act1 expression in Hep G2 whole cell lysate.Application
Western blot analysis of Act1 expression in HeLa nuclear extract .Application
Western blot analysis of Act1 expression in non-transfected control (A) and Act1 siRNA transfected (B) HeLa cells. Blot probed with Act1 (H-300): . α-actinin (H-2): used as specificity and loading control.| Product Name | Act1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Act1 of human origin |
| Modification Notes | Members of the NF˚B family of transcription factors are important in regulating the expression of various cellular and viral genes involved in immune and inflammatory responses, cell survival and stress responses. IL-1, TNFå and other related signaling pathways activate transcription factors through the activation of JNK. The NF˚B signaling pathway converges with the signal-induced activation of JNK upstream of IKK. Isolated from the human embryonic kidney (HEK) 293 cell line, Act1 is an IKK©-associated protein that activates both NF˚B and JNK constitutively. Act1, also designated NF˚B activator 1 or CIKS (for connection to IKK and SAPK/JNK), may function as a coordinator between two stress-induced signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Act1 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Act1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Act1 of human origin |
| Entrez Gene | 10758 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of Act1 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of Act1 expression in HeLa nuclear extract .
Application
Western blot analysis of Act1 expression in non-transfected control (A) and Act1 siRNA transfected (B) HeLa cells. Blot probed with Act1 (H-300): . α-actinin (H-2): used as specificity and loading control.| Application Notes | recommended for detection of Act1 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
