Application
Western blot analysis of CASK expression in mouse brain tissue extract.Application
Western blot analysis of CASK expression in c4 whole cell lysate.Application
Western blot analysis of CASK expression in rat brain tissue extract (A) and DU 145 whole cell lysate (B).Application
Immunofluorescence staining of methanol-fixed DU 145 cells showing cytoplasmic localization.| Product Name | CASK Antibody (H-107) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 353-459 mapping to an internal region of CASK of human origin |
| Modification Notes | The MAGUK (membrane-associated guanylate kinase homologs) family of proteins contain multiple protein-binding domains and are involved in cell junction organization, tumor suppression, and signaling. CASK (also designated LIN-2) belongs to a MAGUK subfamily which is characterized by a novel domain structure that consists of a calcium/calmodulin- dependent protein kinase domain followed by PDZ, SH3 and guanylate kinase-like (GUK) domains. CASK is expressed in rat brain where it binds to cell-surface proteins, such as neurexin and syndecan, and is thought to be involved in signaling at neuronal synapses. CASK translocates to the nucleus and interacts with Tbr-1 to form a complex, which binds to a specific DNA sequence (the T-element), and induces the expression of specific genes, including Reelin. CASK displays a transcription regulation function, which appears crucial for cerebrocortical development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CASK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 353-459 mapping to an internal region of CASK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 353-459 mapping to an internal region of CASK of human origin |
| Entrez Gene | 8573 |
|---|
Application
Western blot analysis of CASK expression in mouse brain tissue extract.
Application
Western blot analysis of CASK expression in c4 whole cell lysate.
Application
Western blot analysis of CASK expression in rat brain tissue extract (A) and DU 145 whole cell lysate (B).
Application
Immunofluorescence staining of methanol-fixed DU 145 cells showing cytoplasmic localization.| Application Notes | recommended for detection of CASK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
