Application
Western blot analysis of Endophilin II expression in non-transfected: (A) and human Endophilin II transfected: (B) 293T whole cell lysates.| Product Name | Endophilin II Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 256-315 mapping near the C-terminus of Endophilin II of human origin |
| Modification Notes | The endophilins comprise a family of three SH3 domain-containing proteins designated endophilin I, II and III, or alternatively known as SH3P4, SH3P8 and SH3P13, respectively (1,2). These proteins associate with amphiphysin, synaptojanin and dynamin and are implicated in presynaptic vesicle trafficking at nerve terminals (3). The expression patterns of the endophilins are consistent with their cellular functions at the neuronal synapse as endophilin I is expressed only in the brain (4). Both endophilin II and endophilin III are detected in a variety of tissues (5,6). Endophilin I is also implicated in modulating G-protein coupled receptor signaling by functioning as an adapter protein and directing beta1 adrenergic receptors to the endocytic machinery (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Endophilin II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 256-315 mapping near the C-terminus of Endophilin II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 256-315 mapping near the C-terminus of Endophilin II of human origin |
| Entrez Gene | 6455 |
|---|
Application
Western blot analysis of Endophilin II expression in non-transfected: (A) and human Endophilin II transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Endophilin II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
