Application
Western blot analysis of cathepsin L expression in rat kidney tissue extract.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Western blot analysis of cathepsin L expression in non-transfected control (A) and cathepsin L siRNA transfected (B) NIH/3T3 cells. Blot probed with cathepsin L (H-80): . Actin (I-19): used as specificity and loading control.| Product Name | cathepsin L Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cathepsin L precursor of human origin |
| Modification Notes | The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin L (also designated major excreted protein, MEP or CATL) is a member of the peptidase C1 family and has been identified as a protein that is most closely related to cathepsin H. It is a lysosomal cysteine proteinase that mediates intracellular protein catabolism for collagen, elastin and å-1 protease inhibitor. Cathepsin L is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Transformed mouse fibroblasts stimulated by growth factors or tumor promoters secrete a form of cathepsin L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor cathepsin L of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with precursor cathepsin L2: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cathepsin L precursor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping at the N-terminus of cathepsin L precursor of human origin |
| Entrez Gene | 1514 |
|---|
Application
Western blot analysis of cathepsin L expression in rat kidney tissue extract.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Western blot analysis of cathepsin L expression in non-transfected control (A) and cathepsin L siRNA transfected (B) NIH/3T3 cells. Blot probed with cathepsin L (H-80): . Actin (I-19): used as specificity and loading control.| Application Notes | recommended for detection of precursor cathepsin L of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with precursor cathepsin L2: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
