Application
Western blot analysis of Glucosidase IIβ expression in non-transfected 293T: (A), mouse Glucosidase IIβ transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Glucosidase IIβ expression in non-transfected: (A) and human Glucosidase IIβ transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Glucosidase IIβ expression in Jurkat (A) and HeLa (B) whole cell lysates.| Product Name | Glucosidase IIβ Antibody (H-195) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 333-527 of Glucosidase IIβ of human origin |
| Modification Notes | Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex. Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus. The phosphorylated form of Glucosidase IIb is localized in the plasma membrane and is highly expressed in FGF-stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIb was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIb retains the complete complex in the ER. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of the β subunit of Glucosidase II of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 333-527 of Glucosidase II&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 333-527 of Glucosidase IIβ of human origin |
| Entrez Gene | 5589 |
|---|
Application
Western blot analysis of Glucosidase IIβ expression in non-transfected 293T: (A), mouse Glucosidase IIβ transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Glucosidase IIβ expression in non-transfected: (A) and human Glucosidase IIβ transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Glucosidase IIβ expression in Jurkat (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of the β subunit of Glucosidase II of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
