Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of FTα expression in A-431 whole cell lysate.Application
Western blot analysis of FTFTα expression in non-transfected: (A) and human FTα transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane, cytoplasmic, and nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of FTα expression in A-431 (A) and Jurkat (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.Application
Western blot analysis of FTα expression in non-transfected: (A) and human FTα transfected: (B) 293T whole cell lysates.| Product Name | FTα Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of FTα of human origin |
| Modification Notes | Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical a and b subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an ab heterodimer. The b subunit binds the peptide substrate while the a subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate. The a subunit is shared with a second prenyltransferase, geranylgeranyl transferase, that attaches 20 carbon geranylgeranyl to Ras-related proteins that terminate in a Cys-A-A-Xaa recognition site in which Xaa is leucine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FTα of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of FT&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of FTα of human origin |
| Entrez Gene | 2339 |
|---|
Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of FTα expression in A-431 whole cell lysate.
Application
Western blot analysis of FTFTα expression in non-transfected: (A) and human FTα transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing membrane, cytoplasmic, and nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of FTα expression in A-431 (A) and Jurkat (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.
Application
Western blot analysis of FTα expression in non-transfected: (A) and human FTα transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FTα of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
