Application
Western blot analysis of TRAF4 expression in non-transfected 293T: (A), human TRAF4 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane and cytoplasmic staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TRAF4 expression in HeLa (A), A-431 (B) and SK-BR-3 (C) whole cell lysates and PMA-treated A-431 (D) and SK-BR-3 (E) nuclear extracts.Application
Western blot analysis of human recombinant TRAF4 fusion protein.| Product Name | TRAF4 Antibody (H-72) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 40-111 of TRAF4 of human origin |
| Modification Notes | The tumor necrosis factor family (TNF) receptor superfamily is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine-rich extracellular domains. Members of this family include TNF-RI, TNF-RII and CD40 (1-3). Ligands for these receptors can be small, secreted proteins such as TNF or type II integral membrane proteins as is the case for the CD40 ligand, CD40L (3,4). While the signal transduction mechanism of the TNF receptor superfamily is poorly understood, activation of TNF-R or CD40 has been shown to induce the nuclear translocation of NFkB (5,6). Members of the TRAF (TNF receptor-associated factor) family have been implicated in this process (5,6). Four members have thus far been described and are designated TRAF1, TRAF2, TRAF3 (variously referred to as CRAF1, LAP1 or CD40bp) and TRAF4 (7). TRAF4, originally termed CART1, is specifically expressed in breast carcinomas, and is localized to the nucleus in such tissues (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TRAF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 40-111 of TRAF4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 40-111 of TRAF4 of human origin |
| Entrez Gene | 9618 |
|---|
Application
Western blot analysis of TRAF4 expression in non-transfected 293T: (A), human TRAF4 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane and cytoplasmic staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TRAF4 expression in HeLa (A), A-431 (B) and SK-BR-3 (C) whole cell lysates and PMA-treated A-431 (D) and SK-BR-3 (E) nuclear extracts.
Application
Western blot analysis of human recombinant TRAF4 fusion protein.| Application Notes | recommended for detection of TRAF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
