Application
Western blot analysis of Tie-2 expression in HUV-EC-C whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Tie-2 expression in ECV304 whole cell lysate.Application
Western blot analysis of human recombinant Tie-2 fusion protein.| Product Name | Tie-2 Antibody (H-176) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-200 mapping within the extracellular domain of Tie-2 of human origin |
| Modification Notes | Receptor tyrosine kinases play key roles in signal transduction across cell surfaces in biological systems, including the vascular system. These receptors comprise a large and diverse family of catalytically related proteins that, on the basis of sequence and structural similarities, can be divided into several different evolutionary subfamilies. The cloning and characterization of Tie-1 (also designated Tie), a novel human endothelial cell surface receptor tyrosine kinase, has been reported. The extracellular domain of the predicted Tie-1 protein product has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. An additional member of this family has been identified as Tie-2 (also designated Tek). Tie-1 and Tie-2 have been shown to be encoded by distinct genes and to represent members of a new class of receptor tyrosine kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tie-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-200 mapping within the extracellular domain of Tie-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-200 mapping within the extracellular domain of Tie-2 of human origin |
| Entrez Gene | 7010 |
|---|
Application
Western blot analysis of Tie-2 expression in HUV-EC-C whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Tie-2 expression in ECV304 whole cell lysate.
Application
Western blot analysis of human recombinant Tie-2 fusion protein.| Application Notes | recommended for detection of Tie-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
