| Product Name | Asf1 Antibody (FL-279) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-279 representing full length Asf1 of Saccharomyces cerevisiae origin |
| Modification Notes | CIA, an interactor of the CCG1 histone acetyltransferase subunit of TFIID, is a human histone chaperone. The Saccharomyces cerevisiae orthologue Asf1 (anti-silencing function 1) is involved in DNA repair response. Asf1, when overexpressed, causes de-repression of silent loci. Asf1 (also known as Asf1p) interacts with Bdf1p (bromodomain factor 1), which serves as the missing bromodomain in yTAF(II)145. Cell death in S. cerevisiae occurs with a phenotype that largely resembles apoptosis in multicellular organisms, but also has some features of passive cell death (necrosis). Deletion of Asf1 inhibits the normal assembly/disassembly of nucleosomes in yeast and thereby initiates the active cell death system. Yeast CAF-I and Asf1 cooperate to form nucleosomes in vitro . In vivo, Asf1 and Hir proteins physically interact and together promote heterochromatic gene silencing in a manner requiring PCNA. Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Asf1 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-279 representing full length Asf1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-279 representing full length Asf1 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Asf1 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
