Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of CEP55 expression in HeLa (A), Caki-1 (B) and ES-2 (C) whole cell lysates.Application
Western blot analysis of CEP55 expression in Hep G2 whole cell lysate.| Product Name | CEP55 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CEP55 of human origin |
| Modification Notes | CEP55 (Centrosomal protein of 55 kDa), also known as URCC6 (Up-regulated in colon cancer 6), is a 464 amino acid protein that localizes to the centrosome during interphase and may be found throughout the cell during mitosis. Widely expressed with highest expression in testis and lower expression in thymus, bone marrow, placenta, fetal heart, digestive tract and several carcinomas, CEP55 exists as a homodimer that interacts with centrosome components and is involved in mitotic exit and cytokinesis. Human CEP55 undergoes several phosphorylation events throughout the cell cycle, most of which are necessary for proper CEP55 function. Mutations or defects in the gene encoding CEP55 result in a failure to exit mitosis and may be associated with tumor progression. Two isoforms of CEP55 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CEP55 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CEP55 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CEP55 of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of CEP55 expression in HeLa (A), Caki-1 (B) and ES-2 (C) whole cell lysates.
Application
Western blot analysis of CEP55 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of CEP55 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
