Application
Western blot analysis of CKAP5 expression in MDA-MB-468 whole cell lysate.| Product Name | CKAP5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CKAP5 of human origin |
| Modification Notes | CKAP5 (cytoskeleton-associated protein 5), also known as ch-TOG (colonic hepatic tumor overexpressed gene), is a 2,032 amino acid protein that is a member of the TOG/XMAP215 family and contains nine heat repeats. CKAP5 is expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas and is overexpressed in hepatomas and colonic tumors. During interphase, CKAP5 localizes to the perinuclear cytoplasm and the spindle poles, where it plays an important role in spindle microtubule organization. Specifically, CKAP5 is required for extrapole formation in prometaphases lacking Ark-1, a mitotic centrosomal kinase that functions as a spindle regulator and is overexpressed in tumors.. CKAP5 binds to hnRNP A2 (heterogeneous nuclear ribonucleoprotein) and interacts with TACC1, TACC3 and Tubulin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CKAP5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CKAP5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CKAP5 of human origin |
Application
Western blot analysis of CKAP5 expression in MDA-MB-468 whole cell lysate.| Application Notes | recommended for detection of CKAP5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
