Application
Western blot analysis of GRIP-1 expression in K-562 (A), HeLa (B) and PMA-treated HeLa (C) nuclear extracts.Application
Western blot analysis of His-tagged mouse recombinant GRIP-1.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.Application
Control Input (A). Antibodies used included SRC-1 (M-341): , SRC-1 (C-20): and SRC-1 (M-20): (B), Grip-1 (M-434): (C), NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): and NCoA-3 (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.| Product Name | GRIP-1 Antibody (M-343) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 787-1129 mapping within an internal region of GRIP-1 of mouse origin |
| Modification Notes | Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormone-responsive elements. This family includes GRIP-1 (for GR interacting protein-1, also designated NCoA-2 or Tif2); SRC-1 (for steroid receptor coactivator-1, also designated NCoA-1); RAC3 (also designated AIB1, for amplified in breast cancer, or ACTR), which displays elevated expression in estrogen receptor positive ovarian and breast cancers; and p/CIP (for p300/CBP/Co-Integrator Protein), which is required for the transcriptional activation of p300/CBP-dependent transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of GRIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 787-1129 mapping within an internal region of GRIP-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 787-1129 mapping within an internal region of GRIP-1 of mouse origin |
| Entrez Gene | 10499 |
|---|
Application
Western blot analysis of GRIP-1 expression in K-562 (A), HeLa (B) and PMA-treated HeLa (C) nuclear extracts.
Application
Western blot analysis of His-tagged mouse recombinant GRIP-1.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Application
Control Input (A). Antibodies used included SRC-1 (M-341): , SRC-1 (C-20): and SRC-1 (M-20): (B), Grip-1 (M-434): (C), NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): and NCoA-3 (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.| Application Notes | recommended for detection of GRIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
