Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Arnt 1 expression in non-transfected: (A) and mouse Arnt 1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain showing nuclear localization.Application
Antibodies tested include Arnt 1 (N-19): (A,B) and Arnt 1 (H-172): (C,D).| Product Name | Arnt 1 Antibody (H-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin |
| Modification Notes | AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 1 (aryl hydrocarbon receptor nuclear translocator), also known as HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta ot ARNT, is a 789 amino acid nuclear protein that contains a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Arnt 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin |
| Entrez Gene | 405 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Arnt 1 expression in non-transfected: (A) and mouse Arnt 1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain showing nuclear localization.
Application
Antibodies tested include Arnt 1 (N-19): (A,B) and Arnt 1 (H-172): (C,D).| Application Notes | recommended for detection of Arnt 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
