Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Stat1 p84/p91 expression in non-transfected 293T: (A), mouse Stat1 p84/p91 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Stat1 p84/p91 expression in non-transfected 293T: (A), mouse Stat1 p84/p91 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
FCM analysis of LPS-treated BAlb/c cells. Kindly provided by Dr. Subhashini Arimilli at Wake Forest University Baptist Medical Center.Application
Antibodies include Stat1α p91 (C-24): (AC) and Stat1 p84/p91 (E-23): (DF).| Product Name | Stat1 p84/p91 Antibody (E-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of Stat1 p84/p91 of human origin |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-å and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of Stat1β p84 and Stat1α p91 of mouse, rat, human and Xenopus origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 FACS:1:100-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of Stat1 p84/p91 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of Stat1 p84/p91 of human origin |
| Entrez Gene | 6772 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Stat1 p84/p91 expression in non-transfected 293T: (A), mouse Stat1 p84/p91 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Stat1 p84/p91 expression in non-transfected 293T: (A), mouse Stat1 p84/p91 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
FCM analysis of LPS-treated BAlb/c cells. Kindly provided by Dr. Subhashini Arimilli at Wake Forest University Baptist Medical Center.
Application
Antibodies include Stat1α p91 (C-24): (AC) and Stat1 p84/p91 (E-23): (DF).| Application Notes | recommended for detection of Stat1β p84 and Stat1α p91 of mouse, rat, human and Xenopus origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 FACS:1:100-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
