Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of AdipoR1 expression in rat skeletal muscle tissue extract.| Product Name | AdipoR1 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 275-314 mapping near the C-terminus of AdipoR1 of human origin |
| Modification Notes | Adiponectin is a circulating hormone secreted by adipocytes that improves the metabolism of glucose and lipids, and is expressed at low levels in those with obesity and diabetes. Adiponectin receptors AdipoR1 and AdipoR2, also designated progestin and adipoQ receptor family members I and II, respectively, regulate fatty acid oxidation and the uptake of glucose by adiponectin. Each receptor activates a unique set of signaling molecules including AMPK, p38 MAPK and PPARå. AdipoR1 has a high-affinity for globular adiponectin and low-affinity for full-length adiponectin, while AdipoR2 has an intermediate affinity for both forms. AdipoR1 and AdipoR2 are mainly expressed in liver and muscle. Adiponectin, AdipoR1 and AdipoR2 are all associated with body composition, Insulin sensitivity, and metabolic parameters. Physical training increases circulating adiponectin and mRNA expression of AdipoR1 and AdipoR2 in muscle, which may mediate the improvement of Insulin resistance and the metabolic syndrome in response to exercise. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AdipoR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 275-314 mapping near the C-terminus of AdipoR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 275-314 mapping near the C-terminus of AdipoR1 of human origin |
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of AdipoR1 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of AdipoR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
