Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of human recombinant TWEAK fusion protein.| Product Name | TWEAK Antibody (FL-249) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-249 representing full length TWEAK of human origin |
| Modification Notes | Proteins belonging to the tumor necrosis factor (TNF) superfamily are potent mediators of inflammation and of the immune system. Members of the TNF superfamily include TNFb, lymphotoxin b (LTb), CD154 (CD40L), CD30L, CD27L, OX40L, 4-1BBL, FAS-L (APO-1) and TRAIL. Most TNF family members are type II transmembrane proteins that are proteolytically processed at their carboxy terminal extracellular domain to form a soluble homotrimeric molecule. TWEAK (also designated Apo-3L) has been identified as a secreted ligand belonging to the TNF superfamily. TWEAK seems to induce apoptosis weakly, and it may be involved in cell differentiation in vivo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of TWEAK of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-249 representing full length TWEAK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-249 representing full length TWEAK of human origin |
| Entrez Gene | 8742 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of human recombinant TWEAK fusion protein.| Application Notes | recommended for detection of TWEAK of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
