Application
Western blot analysis of FAS-L expression in Jurkat whole cell lysate.Application
Western blot analysis of FAS-L expression in non-transfected: (A) and human FAS-L transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testes tissue showing cytoplasmic staining of cells in seminiferus ducts.Application
FAS-L expression in MS plaque. Confocal microscopy showing cells labeled by TUNEL (yellow) and (red). Arrows indicate double-labeled cells. Reproduced from P. Dowling et al.,The Journal of Experimental Medicine, 1996, vol: 184, 1513-1518 by copyright permission of the Rockefeller University Press.| Product Name | FAS-L Antibody (Q-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of FAS-L of human origin |
| Modification Notes | Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FAS-L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of FAS-L of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of FAS-L of human origin |
| Entrez Gene | 356 |
|---|
Application
Western blot analysis of FAS-L expression in Jurkat whole cell lysate.
Application
Western blot analysis of FAS-L expression in non-transfected: (A) and human FAS-L transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testes tissue showing cytoplasmic staining of cells in seminiferus ducts.
Application
FAS-L expression in MS plaque. Confocal microscopy showing cells labeled by TUNEL (yellow) and (red). Arrows indicate double-labeled cells. Reproduced from P. Dowling et al.,The Journal of Experimental Medicine, 1996, vol: 184, 1513-1518 by copyright permission of the Rockefeller University Press.| Application Notes | recommended for detection of FAS-L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
