Application
Western blot analysis of NgR3 expression in U-87 MG (A) and MCF7 (B) whole cell lysates and rat brain (C) and mouse brain (D) tissue extracts.| Product Name | NgR3 Antibody (H-144) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NgR3 (Nogo-66 receptor-related protein 3), also known as RTN4RL1 (reticulon 4 receptor-like 1), NGRH2 or NGRL2 (Nogo receptor-like 2), is a 441 amino acid protein that belongs to the Nogo receptor family. Localized to the cell membrane, NgR3 contains 8 LRR (leucine-rich) repeats. NgR3 is predominantly expressed in brain, where it localizes to the surface of neurons, but is also found at lower levels in lung, placenta, kidney, mammary gland, skeletal muscle, spleen and salivary gland. NgR3 is thought to play a role in the regulation of axonal regeneration and plasticity in the adult central nervous system. The gene that encodes NgR3 maps to human chromosome 17, which makes up over 2.5% of the human genome with about 81 million bases encoding over 1,200 genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NgR3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with NgR2 also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 277-420 mapping near the C-terminus of NgR3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 277-420 mapping near the C-terminus of NgR3 of human origin |
| Entrez Gene | 146760 |
|---|
Application
Western blot analysis of NgR3 expression in U-87 MG (A) and MCF7 (B) whole cell lysates and rat brain (C) and mouse brain (D) tissue extracts.| Application Notes | recommended for detection of NgR3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with NgR2 also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
