Application
Western blot analysis of DDX33 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).Application
Western blot analysis of DHX33 expression in non-transfected: (A) and mouse DHX33 transfected: (B) 293T whole cell lysates.| Product Name | DDX33 Antibody (S-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX33 (DEAD (Asp-Glu-Ala-His) box polypeptide 33), also known as DHX33, is a 707 amino acid nucleolar protein belonging to the DEAD box helicase family. Containing a helicase ATP-binding domain and a helicase C-terminal domain, DDX33 is encoded by a gene located on human chromosome 17. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two isoforms of DDX33 exists due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDX33 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other DDX family members also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of DDX33 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of DDX33 of human origin |
| Entrez Gene | 56919 |
|---|
Application
Western blot analysis of DDX33 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).
Application
Western blot analysis of DHX33 expression in non-transfected: (A) and mouse DHX33 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of DDX33 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other DDX family members also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
