Application
Western blot analysis of CapZ-α expression in A-431 (A) and T24 (B) whole cell lysates.| Product Name | CapZ-α Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 157-286 mapping at the C-terminus of CapZ-α1 of human origin |
| Modification Notes | The F-Actin family of capping proteins includes CapZ-å1, CapZ-å2, CapZ-å3 and CapZ-∫3, all of which function in a calcium-dependent manner and bind to the fast growing barbed end of Actin filaments, thereby blocking protein exchange at these ends. The F-Actin capping protein complex is a heterodimer consisting of å and ∫ subunits that caps the barbed ends of Actin filaments and nucleates the polymerization of Actin monomers, yet does not sever Actin filaments. CapZ-å1, also known as F-Actin-capping protein subunit alpha-1, is a 286 amino acid subunit of the heterodimer that forms the F-Actin capping protein complex. CapZ-å1 also has been shown to bind S-100 ∫ chain, a signaling molecule involved in the calcium-sensitive assembly of intermediate filaments that has been linked to Alzheimer’s disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CapZ-α1 and CapZ-α2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 157-286 mapping at the C-terminus of CapZ-&alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 157-286 mapping at the C-terminus of CapZ-α1 of human origin |
| Entrez Gene | 829 |
|---|
Application
Western blot analysis of CapZ-α expression in A-431 (A) and T24 (B) whole cell lysates.| Application Notes | recommended for detection of CapZ-α1 and CapZ-α2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
