Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoskeletal staining.Application
Western blot analysis of Filaggrin expression in F9 (A), NIH/3T3 (B) and c4 (C) whole cell lysates and mouse embryo tissue extract (D).| Product Name | Filaggrin Antibody (M-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 11-300 mapping near the N-terminus of Filaggrin of mouse origin |
| Modification Notes | Profilaggrin is a large, insoluble, highly phosphorylated precursor protein containing several tandem copies of a 324 amino acid stretch. Mammalian profilaggrin is a major protein component of keratohyalin granules in the living cells of the epidermis. Keratohyalin granules contribute to the keratin content of dead cornified cells. During terminal differentiation of the epidermis, profilaggrin is proteolytically processed into active filaggrin molecules that promote aggregation and disulfide-bond formation of keratin intermediate filaments. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Filaggrin of mouse origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 11-300 mapping near the N-terminus of Filaggrin of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 11-300 mapping near the N-terminus of Filaggrin of mouse origin |
| Entrez Gene | 14246 |
|---|
Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoskeletal staining.
Application
Western blot analysis of Filaggrin expression in F9 (A), NIH/3T3 (B) and c4 (C) whole cell lysates and mouse embryo tissue extract (D).| Application Notes | recommended for detection of Filaggrin of mouse origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
