Application
Western blot analysis of MKP-7 expression in HeLa (A) and Jurkat (B) whole cell lysates.| Product Name | MKP-7 Antibody (H-44) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DUSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DUSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. MKP-7 binds to and inactivates p38 MAPK isoforms alpha and beta, and JNK/SAPK, but not ERK. ERK phosphorylates MKP-7 on Ser 446, thereby stabilizing the protein and blocking JNK activation. MKP-7 is predominantly localized in the cytoplasm, but becomes exclusively nuclear following leptomycin B treatment. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MKP-7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 501-544 mapping near the C-terminus of MKP-7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 501-544 mapping near the C-terminus of MKP-7 of human origin |
| Entrez Gene | 80824 |
|---|
Application
Western blot analysis of MKP-7 expression in HeLa (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of MKP-7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
