Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing cytoplasmic staining of cells in seminiferus ducts & Leydig cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing cytoplasmic staining of cells in seminiferus ducts & Leydig cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SDHA expression in mouse heart (A) and rat heart (B) tissue extracts.| Product Name | SDHA Antibody (H-204) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature SDHA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 461-664 mapping at the C-terminus of succinate dehydrogenase flavoprotein subunit of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 461-664 mapping at the C-terminus of succinate dehydrogenase flavoprotein subunit of human origin |
| Entrez Gene | 6389 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing cytoplasmic staining of cells in seminiferus ducts & Leydig cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing cytoplasmic staining of cells in seminiferus ducts & Leydig cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SDHA expression in mouse heart (A) and rat heart (B) tissue extracts.| Application Notes | recommended for detection of precursor and mature SDHA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
