Application
Western blot analysis of Amphiregulin expression in non-transfected 293T: (A), mouse Amphiregulin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | Amphiregulin Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Epidermal growth factor (EGF) family member Amphiregulin was initially characterized as a schwannoma-derived growth factor (SDGF) that was expressed in response to androgen in the SC2G murine cell line. Amphiregulin has subsequently been characterized as an important growth factor for normal human keratinocyte proliferation. Amphiregulin is produced and secreted by keratinocytes and acts as an autocrine growth factor. Amphiregulin binds ErbB-1, which is essential for epithelial development in the skin, lung and gastrointestinal tract. Withdrawl of Amphiregulin has been shown to result in down regulation of telomerase activity in human keratinocytes and this suggests that Amphiregulin plays a role in cell senescence. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Amphiregulin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-155 of Amphiregulin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-155 of Amphiregulin of human origin |
| Entrez Gene | 374 |
|---|
Application
Western blot analysis of Amphiregulin expression in non-transfected 293T: (A), mouse Amphiregulin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of Amphiregulin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
