Application
Western blot analysis of TDP2 expression in non-transfected: (A) and human TDP2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TDP2 expression in non-transfected: (A) and human EAPII transfected: (B) 293 whole cell lysates.| Product Name | TDP2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER81, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5’-GGAA-3’ element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation. The Ets-1-associated protein II (EAPII) interacts with Ets-1 as a negative modulator of Ets-1 transcriptional activity. EAPII predominantly localizes to the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TDP2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-362 mapping at the C-terminus of TDP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-362 mapping at the C-terminus of TDP2 of human origin |
| Entrez Gene | 51567 |
|---|
Application
Western blot analysis of TDP2 expression in non-transfected: (A) and human TDP2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TDP2 expression in non-transfected: (A) and human EAPII transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of TDP2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
