Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of a subset of myocytes.Application
Western blot analysis of AK1 expression in non-transfected 293T: (A), human AK1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed L6 cells showing cytoplasmic localization.Application
Western blot analysis of AK1 expression in L6 whole cell lysate.Application
Western blot analysis of AK1 expression in rat skeletal muscle tissue extract.| Product Name | AK1 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Adenylate kinases 1–5 (designated AK1–5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling, by catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP). Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia. Human AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes and is clustered within myofibrils or bound to membranes. AK1-mediated phosphotransfer is essential for maintaining sufficient cellular energy, which enables proper skeletal muscle performance and metabolic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 105-194 mapping at the C-terminus of AK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 105-194 mapping at the C-terminus of AK1 of human origin |
| Entrez Gene | 203 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of a subset of myocytes.
Application
Western blot analysis of AK1 expression in non-transfected 293T: (A), human AK1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed L6 cells showing cytoplasmic localization.
Application
Western blot analysis of AK1 expression in L6 whole cell lysate.
Application
Western blot analysis of AK1 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of AK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
