Application
Western blot analysis of full length human recombinant Wnt-5a/b fusion protein.| Product Name | Wnt-5a/b Antibody (H-104) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 101-204 mapping within an internal region of Wnt-5b of human origin |
| Modification Notes | Belonging to a family of protooncogenes, Wnt proteins include at least 13 known members that are expressed in species ranging from Drosophila to man. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and functions as a regulator of hematopoietic stem cell function. Wnt-5b is a 359 amino acid secreted protein that localizes to the extracellular matrix. Thought to function as a signaling molecule, Wnt-5b interacts with PORCN and acts as a ligand for members of the frizzled family of transmembrane receptors, possibly affecting the development of discrete tissue regions. Wnt-5b is expressed in a variety of tissues, including fetal brain, kidney, lung, ovary and small intestine, as well as in gastric cancer and teratocarcinoma cell lines, suggesting a role for Wnt-5b in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Wnt-5a and Wnt-5b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-204 mapping within an internal region of Wnt-5b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-204 mapping within an internal region of Wnt-5b of human origin |
Application
Western blot analysis of full length human recombinant Wnt-5a/b fusion protein.| Application Notes | recommended for detection of Wnt-5a and Wnt-5b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
