Application
Western blot analysis of ADX Reductase expression in non-transfected: (A) and human ADX Reductase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ADX Reductase expression in non-transfected 293T: (A), human ADX Reductase transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ADX Reductase expression in SW13 (A) and Hep G2 (B) whole cell lysates and rat adrenal gland tissue extract (C).Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Product Name | ADX Reductase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 192-491 mapping at the C-terminus of Adrenodoxin Reductase of human origin |
| Modification Notes | Adrenodoxin Reductase (ADX Reductase) is a mitochondrial flavoprotein that receives electrons from NADPH and thereby initiates the electron-transport chain serving mitochondrial cytochromes P450. ADX Reductase participates in cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-? hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney and sterol C-27 hydroxylation in the liver. Alternate splicing of ADX Reductase produces two isoforms. Human ADX Reductase maps to human chromosome 17q24-q25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Adrenodoxin Reductase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 192-491 mapping at the C-terminus of Adrenodoxin Reductase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 192-491 mapping at the C-terminus of Adrenodoxin Reductase of human origin |
| Entrez Gene | 2232 |
|---|
Application
Western blot analysis of ADX Reductase expression in non-transfected: (A) and human ADX Reductase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ADX Reductase expression in non-transfected 293T: (A), human ADX Reductase transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ADX Reductase expression in SW13 (A) and Hep G2 (B) whole cell lysates and rat adrenal gland tissue extract (C).
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Adrenodoxin Reductase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
