Application
Western blot analysis of UCH-L5 expression in non-transfected: (A) and mouse UCH-L5 transfected: (B) CHO whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of UCH-L5 expression in non-transfected: (A) and human UCH-L5 transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Product Name | UCH-L5 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 220-329 mapping at the C-terminus of UCH-L5 of human origin |
| Modification Notes | As a component of the 19S regulatory complex of the 26S proteosome, UCH-L5 (ubiquitin carboxyl-terminal hydrolase isozyme L5), also known as UCH37, is a 329 amino acid protein that functions to edit polyubiquinated protein substrates. Since UCH-L5 has the potential to rescue ubiquinated proteins, including oncogenic proteins, from proteasomal degradation, it is likely that deregulation of UCH-L5 may affect tumor growth. Through associations with Smad7, UHC-L5 can dramatically upregulate TGF∫-dependent gene expression by de-ubiquinating and stabilizing TGF∫ RI. Also, since overexpression of UCH-L5 and other deubiquitinating enzymes has been observed in many cancer cell lines, inhibition of these proteins may be of some interest in designing therapies for cancer treatment. There are four isoforms of UCH-L5 that exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UCH-L5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 220-329 mapping at the C-terminus of UCH-L5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 220-329 mapping at the C-terminus of UCH-L5 of human origin |
| Entrez Gene | 51377 |
|---|
Application
Western blot analysis of UCH-L5 expression in non-transfected: (A) and mouse UCH-L5 transfected: (B) CHO whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of UCH-L5 expression in non-transfected: (A) and human UCH-L5 transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of UCH-L5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
