Application
Western blot analysis of UBA3 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | UBA3 Antibody (E-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins (1-3). The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation (1-3). In the yeast, Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Ubc12 and Ubc9 (4-5). Rub1 shares 53% homology with ubiquitin and requires activation via Ula1, Uba3 and Ubc12 in order to conjugate to substrates directed to different proteolytic systems (4). Skp1 connects cell cycle regulators to the ubiquitin proteolysis machinery (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of UBA3 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of UBA3 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9039 |
|---|
Application
Western blot analysis of UBA3 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
