Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of LIP5 expression in non-transfected 293T: (A), human LIP5 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | LIP5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | LIP5 (LYST-interacting protein 5), also known as VTA1 (Vps20-associated 1 homolog), DRG1 (dopamine-responsive protein DRG-1), SBP1 (SKD1 binding protein 1) or My012, is a 307 amino acid protein belonging to the VTA1 family. LIP5 plays a role in sorting membrane proteins, including lysosomal enzymes, lipids and stimulated growth factor receptors, for lysosomal degradation in a pathway known as the endosomal multivesicular bodies (MVB) pathway. Found in brain, liver, heart and kidney, LIP5 localizes to cytoplasm and both endosomal and peripheral membranes. RNAi studies demonstrate that LIP5 depletion decreases human immunodeficiency virus type 1 (HIV-1) budding, and LIP5 is known to interact with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIP5 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of LIP5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of LIP5 of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of LIP5 expression in non-transfected 293T: (A), human LIP5 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of LIP5 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
