| Product Name | Herc1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | One of the largest human proteins, Herc1, also designated p532 or p619, is a 4861 amino acid protein that contains a variety of functional domains, including WD repeats, RCC1 repeats, beta-repeat domains and one HECT domain. Herc1 is ubiquitously expressed in human and mouse tissues and is overexpressed in several human tumor cell lines. It localizes to the cytosol and the Golgi apparatus, where it acts as a guanine nucleotide exchange factor on ARF1 and Rab proteins to mediate membrane trafficking. Herc1 also is involved in proliferation and growth through its interactions with clathrin, M2-pyruvate kinase and TSC2 proteins. Mutations in the TSC2 protein allow for binding to Herc1 in the presence of TSC1, which destabilizes the TSC2 protein. This results in tuberous sclerosis complex (TSC), an autosomal dominant disease characterized by hamartoma formation in various organs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Herc1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 591-890 mapping near the N-terminus of Herc1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 591-890 mapping near the N-terminus of Herc1 of human origin |
| Application Notes | recommended for detection of Herc1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
