Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of MAP-2 expression in non-transfected: (A) and truncated mouse MAP-2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of MAP-2 low molecular weight isoform expression in IMR-32 (A) and SK-N-SH (B) whole cell lysates.| Product Name | MAP-2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MAP-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAP-2 of human origin |
| Entrez Gene | 4133 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of MAP-2 expression in non-transfected: (A) and truncated mouse MAP-2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of MAP-2 low molecular weight isoform expression in IMR-32 (A) and SK-N-SH (B) whole cell lysates.| Application Notes | recommended for detection of MAP-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
