Application
Western blot analysis of TRNT1 expression in IMR-32 whole cell lysate.Application
Western blot analysis of TRNT1 expression in human fetal brain tissue extract.| Product Name | TRNT1 Antibody (J-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRNT1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | TRNT1 (tRNA nucleotidyl transferase, CCA-adding, 1), also known as CCA1, MtCCA or CGI-47, is a 434 amino acid mitochondrial protein belonging to the tRNA nucleotidyltransferase/poly(A) polymerase family. Considered a CCA-adding enzyme, TRNT1 is essential for catalyzing the addition of the CCA terminus to the 3-prime end of tRNA precursors, a reaction which is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis. Existing a three isoforms produced by alternative splicing events, TRNT1 binds manganese as a cofactor and is subject to homodimerization by disulfied linkage. TRNT1 is encoded by a gene located on human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRNT1 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRNT1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 51095 |
|---|
Application
Western blot analysis of TRNT1 expression in IMR-32 whole cell lysate.
Application
Western blot analysis of TRNT1 expression in human fetal brain tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
